Chart showing surface area seeding density cells at confluency and volumes of versene trypsin and medium for various culture dishes plates and flasks. Recommended growth average medium volume approx. Dish diameter actual area calculated area relative to 35 mm dish 35 mm 10 cm2 9 6 cm2 1 60 mm 220 cm 28 cm2 2 100 mm 60 cm 2 78 5 cm 6 actual area.
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